effects of platelet-rich plasma on cartilage grafts in rabbits as an animal model

نویسندگان

ali manafi department of plastic surgery, tehran university of medical sciences, tehran, iran

kamran kaviani far department of plastic surgery, tehran university of medical sciences, tehran, iran

masoud moradi researcher, medical student, tehran university of medical sciences, tehran, iran

amir manafi researcher, medical student, shahid beheshti university of medical sciences, tehran, iran

چکیده

background cartilage tissue has limited regenerative capacity and the management of cartilage defects has always been a challenging issue. platelet-rich plasma (prp) has been recently been used to improve healing of cartilage defects. in the present experiment, we aimed to investigate the effects of prp on regeneration capacity as well as survival of the cartilage grafts in a rabbit model. methods in 15 white new zealand rabbits, auricular cartilage was used to produce 4 pieces of cartilage (each about 2×2 cm). two samples were diced to small pieces and 2 samples left intact. prp was prepared using 5 ml of auricular blood. after measuring of the weight and volume of cartilages, they mixed with either normal saline or prp and then implanted on the back of the rabbit. after 12 weeks, the implants were removed and the weight and volume were measured and the numbers of nucleated lacunae were counted on h & e staining. results histological findings showed that in both the intact and diced cartilages, adding prp resulted in increasing regeneration of chondrocytes. moreover, adding prp to intact cartilages had a significant effect in maintaining the grafts ‘weight and volume (p<0.05) but in diced cartilages, this effect was not significant. conclusion our findings yielded valuable information on the effect of prp on survival and regeneration of autologous cartilage grafts as the extent of angiogenesis and the diameters of vessels were more pronounced in the side using prp and markedly lesser reduction of weight and volume were visible in this group. prp was effective in increasing survival and regeneration capacity of cartilage grafts in rabbit model.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Platelet-Rich Plasma on Cartilage Grafts in Rabbits as an Animal Model

BACKGROUND Cartilage tissue has limited regenerative capacity and the management of cartilage defects has always been a challenging issue. Platelet-rich plasma (PRP) has been recently been used to improve healing of cartilage defects. In the present experiment, we aimed to investigate the effects of PRP on regeneration capacity as well as survival of the cartilage grafts in a rabbit model. M...

متن کامل

Effects of Platelet-Rich Plasma on Cartilage Grafts in Rabbits as an Animal Model

BACKGROUND Cartilage tissue has limited regenerative capacity and the management of cartilage defects has always been a challenging issue. Platelet-rich plasma (PRP) has been recently been used to improve healing of cartilage defects. In the present experiment, we aimed to investigate the effects of PRP on regeneration capacity as well as survival of the cartilage grafts in a rabbit model. ME...

متن کامل

Effects of Platelet-Rich Plasma & Platelet-Rich Fibrin with and without Stromal Cell-Derived Factor-1 on Repairing Full-Thickness Cartilage Defects in Knees of Rabbits

Background: The purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) containing stromal cell-derived factor-1 (SDF1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect.Methods: We created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen matu...

متن کامل

effects of platelet-rich plasma & platelet-rich fibrin with and without stromal cell-derived factor-1 on repairing full-thickness cartilage defects in knees of rabbits

background: the purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (prp) and platelet-rich fibrin (prf) containing stromal cell-derived factor-1 (sdf1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect. methods: we created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen mat...

متن کامل

Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model.

OBJECTIVES/HYPOTHESIS The objective of this study was to compare the viability of cartilage grafts embedded in platelet-rich fibrin matrix (PRFM) wrapped with no material (bare diced cartilage grafts), oxidized methylcellulose (Surgicel), or acellular dermal tissue (AlloDerm). STUDY DESIGN Experimental study. METHODS In this study, six New Zealand rabbits were used. Cartilage grafts includi...

متن کامل

Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat

BACKGROUND Autologous platelet concentrate has been used to improve the function and regeneration of injured tissues. Tendinopathies are common in clinical practice, although long-term treatment is required. On the basis of lead time, we compared the effect of using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in repairing rat Achilles tendon. METHODS The effectiveness of usi...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
world journal of plastic surgery

جلد ۱، شماره ۲، صفحات ۹۱-۹۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023